Approximation of a data transfer speed of a protocol

I have designed and developed a protocol for a progressive file transfer. Now I want to calculate some metrics for the same. The most important one is speed. Currently, I ran both the client and the server on a single machine, hence the speed was say,x. But if the protocol is to be run in a distributed environment with routers, there will be some delays introduced, like processing delay, queue delay, propagation delay, etc. Could some one tell me what are the points I should consider to calculate a rough speed of the protocol. Should it be a function of these delays?

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