How to removed signed trusted app from Mac OSX?

On my Mac OSX machine I ran an app that was correctly signed by my organization, but not downloaded from the app store. Now when I go into Gatekeeper and change the allow apps setting to only the app store the app can still be run. Deleting it and copying it back over to the box didn't have an effect. Where do I go to a clear out the trusted certificate/etc on the system where the app will no longer run with the app store only setting in Gatekeeper.

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